alpha 2 macroglobulin  (Santa Cruz Biotechnology)

Journal: Arthritis Research & Therapy

Article Title: Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis

doi: 10.1186/ar3533

Figure Lengend Snippet: Gene-specific primer sequences used in RT-PCR and quantitative real-time RT-PCR

Article Snippet: Predesigned primers were used for SIRT1 (HA044263; Takarabio, Tokyo, Japan), α 2 -macroglobulin (HA107564; Takarabio), cytokeratin-18 (HA138837; Takarabio) and CD56 (HA106134; Takarabio) [ ].

Techniques: Sequencing

Journal: Proteome Science

Article Title: Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms

doi: 10.1186/1477-5956-11-27

Figure Lengend Snippet: Examination of the decreased level of α-2-macroglobulin in postsurgical sera compared with that in presurgical sera of both AAA and TAA patients, and restoration of its level to that in normal control sera. Western blsot analyses with anti-α-2-macroglobulin antibody in presurgical (pre) and postsurgical (post) sera of 12 patients with AAA ( A ) and 10 patients with TAA ( B ) were performed as indicated by arrows. Representative photos of two each of patients (patient no. #AAA11, #AAA13, #TAA10 and #TAA11) as well as a mixture of 10 normal control sera (N) are shown. To confirm equal levels of proteins per lane, non-specific proteins stained with Coomassie Brilliant Blue are shown (CBB). The intensity of each band in presurgical and postsurgical sera of 12 AAA and 10 TAA patients and 10 normal control sera that reacted with anti-α-2-macroglobulin antibody was measured. Ratios of levels of α-2-macroglobulin in presurgical and postsurgical sera and normal control sera compared with that in the mixture of 10 normal control sera (1.0) were determined by band intensity. Means ± SE of triplicate experiments were calculated, and statistical analysis was performed using the paired t -test between presurgical and postsurgical data, whereas it was done using the unpaired t -test between postsurgical and normal control data. ** p < 0.01.

Article Snippet: The membranes were reacted with rabbit polyclonal anti-fibronectin antibody (Sigma-Aldrich, St. Louis, MO, USA), mouse monoclonal anti-LRG1 antibody (Abnova, Taipei, Taiwan), rabbit polyclonal anti-α-2-macroglobulin antibody (Abcam, Tokyo, Japan), goat polyclonal anti-kallistatin antibody (R & D Systems, Minneapolis, MN, USA), mouse monoclonal anti-gelsolin antibody (Sigma-Aldrich, St. Louis, MO, USA) or goat polyclonal anti-afamin antibody (GeneTex, Irvine, CA, USA).

Techniques: Western Blot, Staining

Journal: Cell chemical biology

Article Title: Dynarrestin, a Novel Inhibitor of Cytoplasmic Dynein

doi: 10.1016/j.chembiol.2017.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Live Cell Imaging of α2M-Labeled Endosome Movement Human α 2 -macroglobulin (α 2 M) (Calbiochem, San Diego, CA) was conjugated with Alexa Fluor 555 using an Invitrogen protein-labeling kit according to the manufacturer’s instructions.

Techniques: Recombinant